Analysis of phospholipid synthesis in mitochondria.

Jorge Montesinos, Estela Area-Gomez, Michael Schlame

Methods in cell biology 2020

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Mitochondria and their associated membranes actively participate in biosynthesis, trafficking, and degradation of cellular phospholipids. Two crucial lipid biosynthetic activities of mitochondria include (i) the decarboxylation of phosphatidylserine to phosphatidylethanolamine and (ii) the de novo synthesis of cardiolipin. Here we describe protocols to measure these two activities, applying isotope-labeled or exogenous substrates in combination with thin-layer chromatography or mass spectrometry. © 2020 Elsevier Inc. All rights reserved.