Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc.

Benjamin J Bowen, Adam R McGarrity, Jenn-Yeu A Szeto, Christopher R Pudney, D Dafydd Jones

Chemical communications (Cambridge, England) 2020 Apr 21

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Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

Citation

Benjamin J Bowen, Adam R McGarrity, Jenn-Yeu A Szeto, Christopher R Pudney, D Dafydd Jones. Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc. Chemical communications (Cambridge, England). 2020 Apr 21;56(31):4308-4311