The Application of Cytokine Expression Assays to Differentiate Active From Previously Treated Syphilis.

To investigate the role of serum cytokine assays to distinguish between active from treated syphilis among serofast patients, we recruited individuals into a prospective cohort study. Participants underwent routine syphilis screening. We selected specimens from a majority cohort of serofast participants with treated and active syphilis. We analyzed specimens with a 62-cytokine multiplex bead-based enzyme-linked immunosorbent assay. Cytokines, brain-derived neurotrophic factor and tumor necrosis factor β, were most predictive. We built a decision tree that was 82.4% accurate, 100% (95% confidence interval, 82%-100%) sensitive, and 45% (18%-75%) specific. Our decision tree differentiated between serum specimens from serofast participants with treated syphilis versus active syphilis. © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Citation

Noah Kojima, Janet C Siebert, Holden Maecker, Yael Rosenberg-Hasson, Segundo R Leon, Silver K Vargas, Kelika A Konda, Carlos F Caceres, Jeffrey D Klausner. The Application of Cytokine Expression Assays to Differentiate Active From Previously Treated Syphilis. The Journal of infectious diseases. 2020 Jul 23;222(4):690-694

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PMID: 32189000

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