Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9.

Ambrin Fatima, Jens Schuster, Talia Akram, Maria Sobol, Jan Hoeber, Niklas Dahl

Stem cell research 2020 Apr

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The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Citation

Ambrin Fatima, Jens Schuster, Talia Akram, Maria Sobol, Jan Hoeber, Niklas Dahl. Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9. Stem cell research. 2020 Apr;44:101758