2D Gel Electrophoresis Analysis of Leishmania Proteomes.

Christina Naula, Richard Burchmore

Methods in molecular biology (Clifton, N.J.) 2020

filter terms:

2D gel electrophoresis enables resolution of intact proteins in complex mixtures and is thus useful for comparative proteomic analysis, particularly of posttranslationally modified proteoforms that might not be distinguished by shotgun proteomic analysis of peptides. 2D gel electrophoresis is a multistep procedure that can require sample-specific optimization. We present a comprehensive protocol that is effective for 2D electrophoretic analysis of proteins from Leishmania promastigotes and may also be employed for Leishmania amastigotes and for trypanosomes.

Citation

Christina Naula, Richard Burchmore. 2D Gel Electrophoresis Analysis of Leishmania Proteomes. Methods in molecular biology (Clifton, N.J.). 2020;2116:577-586