Unique functional properties of slow skeletal muscle tropomyosin.

Alexander M Matyushenko, Daniil V Shchepkin, Galina V Kopylova, Sergey Y Bershitsky, Dmitrii I Levitsky

Biochimie 2020 Jul

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Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein playing an essential role in the regulation of muscle contraction. The α- (Tpm 1.1) and γ- (Tpm 3.12) Tpm isoforms are expressed in fast and slow human skeletal muscles, respectively, while β-Tpm (Tpm 2.2) is expressed in both muscle types. This results in the formation of Tpm αα- and γγ-homodimers as well as αβ- and γβ-heterodimers. The properties of αα-homodimer are well studied, whereas very little is known about the functional properties of γγ-homodimer and γβ-heterodimer. We investigated interaction characteristics of Tpm γγ-homodimer and γβ-heterodimer with actin filaments and Ca2+-regulation of actin-myosin interaction on myosin from fast and slow skeletal muscles. The results showed that complexes formed by γγ-Tpm and γβ-Tpm with F-actin are more stable than those with αα-Tpm and αβ-Tpm. The maximum sliding speed of regulated thin filaments with either γγ-Tpm or γβ-Tpm moving over skeletal myosin was significantly less than that of the filaments with αα-Tpm or αβ-Tpm. The results indicate that isoforms of Tpm along with isoforms of myosin determine of functional properties of skeletal muscles and support an idea on the combined expression of myosin and Tpm isoforms. Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Citation

Alexander M Matyushenko, Daniil V Shchepkin, Galina V Kopylova, Sergey Y Bershitsky, Dmitrii I Levitsky. Unique functional properties of slow skeletal muscle tropomyosin. Biochimie. 2020 Jul;174:1-8