Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles.

Yang Wang, Shulin Du, Ting Liu, Jingyan Ren, Jiayi Zhang, Hao Xu, Hao Zhang, Yi Liu, Laijin Lu

ACS chemical neuroscience 2020 May 06

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Peripheral nerve injuries always cause dysfunction but without ideal strategies to assist the treatment and recovery successfully. The primary way to repair the peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Schwann cells acting as neuroglial cells play a pivotal role during axonal regeneration. The orderly and organized migration of Schwann cells is beneficial for the extracellular matrix connection and Büngner bands formation, which greatly promote the regeneration of axons by offering mechanical support and growth factors. Thus, the use of Schwann cells as therapeutic cells offers us an attractive method for neurorepair therapies, and the ability to direct and manipulate Schwann cell migration and distribution is of great significance in the field of cell therapy in regards to the repair and regeneration of the peripheral nerve. Herein, we design and characterize a type of novel fluorescent-magnetic bifunctional Fe3O4·Rhodamine 6G (R6G)@polydopamine (PDA) superparticles (SPs) and systematically study the biological behaviors of Fe3O4·R6G@PDA SP uptake by Schwann cells. The results demonstrate that our tailor-made Fe3O4·R6G@PDA SPs can be endocytosed by Schwann cells and then highly magnetize Schwann cells by virtue of their excellent biocompatibility. Furthermore, remote-controlling and noninvasive magnetic targeting migration of Schwann cells can be achieved on the basis of the high magnetic responsiveness of Fe3O4·R6G@PDA SPs. At the end, gene expression profile analysis is performed to explore the mechanism of Schwann cells' magnetic targeting migration. The results indicate that cells can sense external magnetic mechanical forces and transduce into intracellular biochemical signaling, which stimulate gene expression associated with Schwann cell migration.

Citation

Yang Wang, Shulin Du, Ting Liu, Jingyan Ren, Jiayi Zhang, Hao Xu, Hao Zhang, Yi Liu, Laijin Lu. Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles. ACS chemical neuroscience. 2020 May 06;11(9):1359-1370