β2 adrenoceptor signaling regulates ion transport in 16HBE14o- human airway epithelial cells.

Rui-Gang Zhang, Chung-Yin Yip, Ke-Wu Pan, Meng-Yun Cai, Wing-Hung Ko

Journal of cellular physiology 2020 Nov

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We investigated the regulation of Cl- secretion by adrenoceptors in polarized 16HBE14o- human bronchial epithelial cells. Treatment with the nonselective β adrenoceptor agonist isoprenaline stimulated an increase in short-circuit current (ISC ), which was inhibited by the β adrenoceptor blocker propranolol. Treatment with procaterol, an agonist specific for the β2 adrenoceptor subtype, stimulated a similar increase in ISC , which was inhibited by the β2 adrenoceptor antagonist ICI 118551. Inhibitors of cystic fibrosis transmembrane conductance regulator (CFTR) and calcium-activated Cl- channel (CaCC), but not K+ channel blockers, were able to inhibit the increase in ISC . "Trimultaneous" recording of ISC and intracellular cyclic adenosine monophosphate (cAMP) and Ca2+ levels in 16HBE14o- epithelia confirmed that the ISC induced by isoprenaline or procaterol involved both cAMP and Ca2+ signaling. Our results demonstrate that β2 adrenoceptors regulate Cl- secretion in the human airway epithelium by activating apical CFTRs and CaCCs via cAMP-dependent and intracellular Ca2+ -dependent mechanisms, respectively. © 2020 Wiley Periodicals, Inc.

Citation

Rui-Gang Zhang, Chung-Yin Yip, Ke-Wu Pan, Meng-Yun Cai, Wing-Hung Ko. β2 adrenoceptor signaling regulates ion transport in 16HBE14o- human airway epithelial cells. Journal of cellular physiology. 2020 Nov;235(11):8387-8401