MiR-155-5p plays as a "janus" in the expression of inflammatory cytokines induced by T-2 toxin.

Pu Guo, Fang Qiao, Deyu Huang, Qinghua Wu, Tianlun Chen, Sara Badawy, Guyue Cheng, Haihong Hao, Shuyu Xie, Xu Wang

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Jun

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Although many studies have shown that inflammatory response plays a crucial role in the various toxic effects of T-2 toxin, there are relatively few reports on the mechanism of this phenomenon. Meanwhile, accumulating evidence has shown that miR-155-5p is activated in the inflammatory response. As molecular pathways and mechanisms involved in T-2 toxin-induced inflammatory response are poorly elucidated, we assessed whether miR-155-5p is involved in the inflammation effects mediated by T-2 toxin. Treatment of RAW264.7 cells with T-2 toxin (14 nM and 12 h) resulted in inflammatory response and associated with alteration of the gene expression signature of miR-155-5p. Knockdown or overexpression of miR-155-5p both indicated that miR-155-5p positively regulated the expression of the inflammation factors. Moreover, bioinformatics prediction and luciferase assay indicated that atg3 and rheb are targets of miR-155-5p. However, atg3 and SOCS1 play positive roles in the inflammatory response regulated by miR-155-5p, while rheb plays a negative role. In addition, the in vivo study showed that single administration of T-2 toxin in mice enhances spleen immune response, which was accompanied by an overexpression of miR-155-5p. These findings indicate that miR-155-5p might have an important role associated with the inflammatory response induced by T-2 toxin. In conclusion, a dual character of miR-155-5p in inflammation response was revealed, which might exist in other reactions in which miR-155-5p is involved. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Pu Guo, Fang Qiao, Deyu Huang, Qinghua Wu, Tianlun Chen, Sara Badawy, Guyue Cheng, Haihong Hao, Shuyu Xie, Xu Wang. MiR-155-5p plays as a "janus" in the expression of inflammatory cytokines induced by T-2 toxin. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2020 Jun;140:111258