Yuping Chen, Gang Zhao, Jakub Zahumensky, Sangeet Honey, Bruce Futcher
Molecular cell 2020 Apr 16Yeast cells must grow to a critical size before committing to division. It is unknown how size is measured. We find that as cells grow, mRNAs for some cell-cycle activators scale faster than size, increasing in concentration, while mRNAs for some inhibitors scale slower than size, decreasing in concentration. Size-scaled gene expression could cause an increasing ratio of activators to inhibitors with size, triggering cell-cycle entry. Consistent with this, expression of the CLN2 activator from the promoter of the WHI5 inhibitor, or vice versa, interfered with cell size homeostasis, yielding a broader distribution of cell sizes. We suggest that size homeostasis comes from differential scaling of gene expression with size. Differential regulation of gene expression as a function of cell size could affect many cellular processes. Copyright © 2020 Elsevier Inc. All rights reserved.
Yuping Chen, Gang Zhao, Jakub Zahumensky, Sangeet Honey, Bruce Futcher. Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast. Molecular cell. 2020 Apr 16;78(2):359-370.e6
PMID: 32246903
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