Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells.

Michi Nakamura, Hidetaka Nishida, Karin Yoshizaki, Hideo Akiyoshi, Shingo Hatoya, Kikuya Sugiura, Toshio Inaba

The Journal of veterinary medical science 2020 May 20

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We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70-80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.

Citation

Michi Nakamura, Hidetaka Nishida, Karin Yoshizaki, Hideo Akiyoshi, Shingo Hatoya, Kikuya Sugiura, Toshio Inaba. Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells. The Journal of veterinary medical science. 2020 May 20;82(5):668-672