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    Nitric oxide (NO) is an important signaling molecule involved in various biological phenomena in many organisms. The physiological functions and metabolism of NO in yeast, a unicellular microorganism, are still unknown, mainly because it is difficult to analyze the intracellular NO levels accurately. Here, we developed a new method of more accurately measuring NO content in yeast cells with the detection limit of 6 nM, by treating the cells with an NO-specific fluorescence probe followed by high-performance liquid chromatography with fluorescence detection (HPLC/FLD). This approach successfully detected and quantified the NO content inside yeast cells treated with an NO donor. Moreover, the HPLC/FLD analysis indicates that the fluorescence induced under some environmental stress conditions, such as ethanol, vanillin, and heat-shock, was not derived from NO. The HPLC/FLD method developed in this study provides a new strategy for measuring the intracellular NO concentration with higher accuracy. Copyright © 2020 Elsevier Inc. All rights reserved.

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    Ryo Nasuno, Seiya Shino, Yuki Yoshikawa, Natsuko Yoshioka, Yuichi Sato, Kohei Kamiya, Hiroshi Takagi. Detection system of the intracellular nitric oxide in yeast by HPLC with a fluorescence detector. Analytical biochemistry. 2020 Jun 01;598:113707

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    PMID: 32251648

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