Correlation Engine 2.0
Clear Search sequence regions

  • amino acids (3)
  • flow (6)
  • improves (1)
  • open (3)
  • peptides (2)
  • trypsin (1)
  • Sizes of these terms reflect their relevance to your search.

    Flow injection chromatography (FIC) or sequential injection chromatography (SIC) is a low-pressure liquid chromatography technique that uses flow injection or sequential injection hardware. Due to the constraints of this hardware, the separation resolution is low; often no more than 3-5 components are resolved. We have recently demonstrated the excellent resolving power of narrow open tubular (OT) columns for various biomolecules, and only moderate elution pressures are needed to carry out these separations. In this paper, we incorporate a narrow OT column with FIC and construct an FIC system using a pressure chamber and two injection valves to implement gradient elution. The resultant system not only improves the resolution but also reduces the system cost. When we use the system to separate peptides from trypsin-digested cytochrome C, we can resolve dozens of peptides (with resolutions of 0.5 or greater) at a speed of 12 samples per hour. When we use this system to separate a mixture containing 3 amino acids, we can base-line resolve these compounds at a speed of 1800 sample per hour. Copyright © 2020 Elsevier B.V. All rights reserved.


    Piliang Xiang, Yu Yang, Zhitao Zhao, Jianhua Wang, Mingli Chen, Apeng Chen, Shaorong Liu. Performing flow injection chromatography using a narrow open tubular column. Analytica chimica acta. 2020 May 01;1109:19-26

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32252901

    View Full Text