Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC lines.

Hannah Döpper, Marius Horstmann, Julia Menges, Morgane Bozet, Deniz Kanber, Laura Steenpass

Stem cell research 2020 May

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Retinoblastoma is a childhood tumor of the retina that is caused mostly by biallelic inactivation of the tumor suppressor gene RB1. To generate a research resource, we abrogated expression of RB1 in H9 hESCs by CRISPR/Cas9 induced deletion of the RB1 promoter, either on one or on both alleles. This enables studies on the role of RB1 loss during differentiation, for example in differentiation towards neural retina. The generation of three isogenic lines per deletion state enables validation of phenotypic results in independent clonal lines. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Citation

Hannah Döpper, Marius Horstmann, Julia Menges, Morgane Bozet, Deniz Kanber, Laura Steenpass. Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC lines. Stem cell research. 2020 May;45:101779