Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification.

Meng Liu, Di Zhang, Xuechong Zhang, Qinfeng Xu, Fei Ma, Chun-Yang Zhang

Chemical communications (Cambridge, England) 2020 May 14

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We develop a label-free fluorescence method for ultrasensitive detection of apoptosis-associated caspase activity based on branched rolling circle amplification. This work demonstrates for the first time the use of a nucleic acid amplification strategy for the amplified detection of caspase activity, which allows for highly sensitive detection of endogenous caspase even at the single-cell level. This method can be further applied for kinetic analysis and inhibitor screening.

Citation

Meng Liu, Di Zhang, Xuechong Zhang, Qinfeng Xu, Fei Ma, Chun-Yang Zhang. Label-free and amplified detection of apoptosis-associated caspase activity using branched rolling circle amplification. Chemical communications (Cambridge, England). 2020 May 14;56(39):5243-5246