Pioneer factors and their in vitro identification methods.

Xinyang Yu, Michael J Buck

Molecular genetics and genomics : MGG 2020 Jul

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Pioneer transcription factors are a special group of transcription factors that can interact with nucleosomal DNA and initiate regulatory events. Their binding to regulatory regions is the first event in gene activation and can occur in silent or heterochromatin regions. Several research groups have endeavored to define pioneer factors and study their binding characteristics using various techniques. In this review, we describe the in vitro methods used to define and characterize pioneer factors, paying particular attention to differences in methodologies and how these differences can affect results.

Citation

Xinyang Yu, Michael J Buck. Pioneer factors and their in vitro identification methods. Molecular genetics and genomics : MGG. 2020 Jul;295(4):825-835