Correlation Engine 2.0
Clear Search sequence regions

  • amines (1)
  • bacteria (1)
  • biocatalysis (3)
  • dna libraries (1)
  • enzymes (1)
  • enzymes (1)
  • past (2)
  • Sizes of these terms reflect their relevance to your search.

    Transaminases (TAms) are important enzymes for the production of chiral amines for the pharmaceutical and fine chemical industries. Novel TAms for use in these industries have been discovered using a range of approaches, including activity-guided methods and homologous sequence searches from cultured microorganisms to searches using key motifs and metagenomic mining of environmental DNA libraries. This mini-review focuses on the methods used for TAm discovery over the past two decades, analyzing the changing trends in the field and highlighting the advantages and drawbacks of the respective approaches used. This review will also discuss the role of protein engineering in the development of novel TAms and explore possible directions for future TAm discovery for application in industrial biocatalysis. KEY POINTS: • The past two decades of TAm enzyme discovery approaches are explored. • TAm sequences are phylogenetically analyzed and compared to other discovery methods. • Benefits and drawbacks of discovery approaches for novel biocatalysts are discussed. • The role of protein engineering and future discovery directions is highlighted.


    Stephen A Kelly, Stefan Mix, Thomas S Moody, Brendan F Gilmore. Transaminases for industrial biocatalysis: novel enzyme discovery. Applied microbiology and biotechnology. 2020 Jun;104(11):4781-4794

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32300853

    View Full Text