Response surface methodology for the extraction of wedelolactone from Eclipta alba using aqueous two-phase extraction.

Neha N Gharat, Virendra K Rathod

Preparative biochemistry & biotechnology 2020

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Aqueous two-phase extraction of wedelolactone from Eclipta alba was studied using the polymer-salt system. The system consisted of polyethylene glycol (PEG) as a top phase (polymer) and sodium citrate as a bottom phase (salt). Process parameters such as PEG concentration, PEG molecular weight, salt concentration, and pH have been optimized using response surface methodology (RSM) with the help of central composite design (CCD). The optimized conditions for aqueous two-phase system (ATPS), in the case of one factor at a time approach, were found as PEG 6000, PEG concentration 18% (w/v), salt concentration 16% (w/v), and pH 7; with maximum extraction yield of 6.52 mg/g. While, RSM studies showed maximum extraction yield of 6.73 mg/g with the optimized parameters as PEG 6000, PEG concentration 18% (w/v), salt concentration 17.96% (w/v), and pH 7. ATPS was found to give a 1.3 fold increase in the extraction yield of wedelolactone as compared to other conventional extraction methods.

Citation

Neha N Gharat, Virendra K Rathod. Response surface methodology for the extraction of wedelolactone from Eclipta alba using aqueous two-phase extraction. Preparative biochemistry & biotechnology. 2020;50(8):827-833