The pathways by which the marine diatom Thalassiosira sp. OUC2 biodegrades p-xylene, combined with a mechanistic analysis at the proteomic level.

Weiyan Duan, Shuhao Du, Fanping Meng, Xiaoling Peng, Lihong Peng, Yufei Lin, Guoshan Wang, Jiangyue Wu

Ecotoxicology and environmental safety 2020 Jul 15

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A marine diatom, Thalassiosira sp. OUC2, was isolated from natural seawater collected from Daya Bay, China. This diatom degraded 1.25-40 mg L-1p-xylene within five days, at a removal efficiency exceeding 98%. Gas chromatography-mass spectrometer (GC-MS) analysis indicated that p-xylene was converted into 4-methylbenzyl alcohol, p-toluic acid, and p-cresol in the presence of strain OUC2. Meanwhile, proteomic analysis showed that, after exposure to p-xylene, several algal enzymes were significantly upregulated: including monooxygenase, alcohol dehydrogenase, benzaldehyde dehydrogenase, benzoate 1,2-dioxygenase, and catechol 2,3-dioxygenase. Moreover, ecotoxicological tests suggested that the intermediate metabolites were less toxic than the parent compound (p-xylene). Thalassiosira sp. OUC2 may thus be suitable for the remediation of p-xylene-contaminated marine environments. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Weiyan Duan, Shuhao Du, Fanping Meng, Xiaoling Peng, Lihong Peng, Yufei Lin, Guoshan Wang, Jiangyue Wu. The pathways by which the marine diatom Thalassiosira sp. OUC2 biodegrades p-xylene, combined with a mechanistic analysis at the proteomic level. Ecotoxicology and environmental safety. 2020 Jul 15;198:110687