Haohao Duan, Mark Donovan, Franck Hernandez, Carmelo Di Primo, Elisabeth Garanger, Xavier Schultze, Sébastien Lecommandoux
Angewandte Chemie (International ed. in English) 2020 Aug 03In this study, an original method of macromolecular design was used to develop a hyaluronidase-1 (HYAL1) inhibitor from its principal substrate, hyaluronic acid (HA). HA-based nanoparticles (HA-NP) were obtained by copolymer self-assembly and their effects on HYAL1 activity were investigated by combining different analytical tools. Compared to HA, HA-NP exhibited an enhanced stability against HYAL1 degradation while maintaining its interaction with the HA receptors CD44 and aggrecan. HA-NP displayed a strong and selective inhibition of HYAL1 activity and retarded the hydrolysis of higher-molar-mass HA in solution. A co-nanoprecipitation process was used to formulate a range of hybrid nanoparticle samples, which demonstrated the specificity and efficiency of HA-NP in HYAL1 inhibition. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Haohao Duan, Mark Donovan, Franck Hernandez, Carmelo Di Primo, Elisabeth Garanger, Xavier Schultze, Sébastien Lecommandoux. Hyaluronic-Acid-Presenting Self-Assembled Nanoparticles Transform a Hyaluronidase HYAL1 Substrate into an Efficient and Selective Inhibitor. Angewandte Chemie (International ed. in English). 2020 Aug 03;59(32):13591-13596
PMID: 32363767
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