Developments of Riboswitches and Toehold Switches for Molecular Detection-Biosensing and Molecular Diagnostics.

Tin Hoang Trung Chau, Dung Hoang Anh Mai, Diep Ngoc Pham, Hoa Thi Quynh Le, Eun Yeol Lee

International journal of molecular sciences 2020 Apr 30

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Riboswitches and toehold switches are considered to have potential for implementation in various fields, i.e., biosensing, metabolic engineering, and molecular diagnostics. The specific binding, programmability, and manipulability of these RNA-based molecules enable their intensive deployments in molecular detection as biosensors for regulating gene expressions, tracking metabolites, or detecting RNA sequences of pathogenic microorganisms. In this review, we will focus on the development of riboswitches and toehold switches in biosensing and molecular diagnostics. This review introduces the operating principles and the notable design features of riboswitches as well as toehold switches. Moreover, we will describe the advances and future directions of riboswitches and toehold switches in biosensing and molecular diagnostics.

Citation

Tin Hoang Trung Chau, Dung Hoang Anh Mai, Diep Ngoc Pham, Hoa Thi Quynh Le, Eun Yeol Lee. Developments of Riboswitches and Toehold Switches for Molecular Detection-Biosensing and Molecular Diagnostics. International journal of molecular sciences. 2020 Apr 30;21(9)