Inhibitory effect of diosmetin on inflammation and lipolysis in coculture of adipocytes and macrophages.

The interaction between adipocytes and macrophages in obese tissues plays a critical role in the onset of metabolic syndromes. This study aimed to evaluate the modulatory effect of diosmetin on anti-inflammatory and anti-lipolytic activities in the coculture of macrophages and adipocytes. The secretion of inflammatory mediators increased in a coculture medium, however, diosmetin significantly reduced the levels of these inflammatory mediators such as nitric oxide (NO), tumor necrosis factor-α, and monocyte chemoattractant protein. Diosmetin down-regulated the protein expression of inducible NO synthase in cocultured macrophages and adipocytes, and inhibited the phosphorylation of mitogen-activated protein kinases and the translocation of p65 and p50 to the nucleus. Moreover, it suppressed the phosphorylation of hormone-sensitive lipase and the production of fatty acid-binding protein 4, and increased the mRNA expression of adiponectin in cocultured adipocytes by 18%-35%. These results indicate that diosmetin inhibited inflammation and lipolysis in the crosstalk between adipocytes and macrophages; diosmetin-containing foods could be used in dietary therapy for the prevention of obesity-related metabolic syndromes. PRACTICAL APPLICATIONS: Diosmetin occurs naturally in citrus fruits that have a high inhibitory effect on inflammation in cocultured adipocytes and macrophages via the inactivation of the MAPKs/NF-kB pathway. Diosmetin also inhibited lipolysis via the reduction of FFA and free glycerol. The present study suggests that treatment of diosmetin may be useful for the prevention of obesity and inflammation-related metabolic syndromes. © 2020 Wiley Periodicals LLC.

Citation

Hana Lee, Jeehye Sung, Younghwa Kim, Heon Sang Jeong, Junsoo Lee. Inhibitory effect of diosmetin on inflammation and lipolysis in coculture of adipocytes and macrophages. Journal of food biochemistry. 2020 Jul;44(7):e13261

Mesh Tags

Substances

PMID: 32367620

search → result