As RNA in situ hybridization (ISH) moves into the mainstream lab and increasingly into clinical adoption and additional multiplexing techniques are developed to enable further RNA ISH identification, a set of guidelines on the validation of ISH is required. These guidelines include choice of methods, appropriate controls, and protocol optimization as well as a central core message of understanding the target, understanding the ISH technique, and using the most appropriate controlling mechanisms to enable reproducible and trustworthy data to be obtained.
Julia Jones, William J Howat. Guidelines for the Optimization and Validation of In Situ Hybridization. Methods in molecular biology (Clifton, N.J.). 2020;2148:3-17
PMID: 32394372
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