BaseSpace
Correlation Engine 2.0
Import Your Data
Literature

FAQ

More FAQs

Back to top
Clear Search sequence regions
(e.g. Hippocampus, Early pregnancy factor, Doxorubicin, rs7903146, FXYD4, Apoptosis, Leukemia)
Bookmark Forward

Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast.

Elisabeth Tamayo, Tamara Gómez-Gallego, Nuria Ferrol

Methods in molecular biology (Clifton, N.J.) 2020


filter terms:
  • amino acid sequence (1)
  • fungal genes (3)
  • fungi (1)
  • gene (2)
  • gene fungal (1)
  • symbiosis (1)
  • yeast (5)
    • Sizes of these terms reflect their relevance to your search.

    The obligate symbiotic nature of arbuscular mycorrhizal (AM) fungi makes extremely difficult their genetic manipulation or transformation. For this reason, a heterologous system has been traditionally used for functional analysis of AM fungal genes, being the budding yeast Saccharomyces cerevisiae an organism suitable for this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation, heterologous gene expression, functional complementation assays, preparation of yeast extracts, and subcellular localization of the encoded protein.

    Citation

    Elisabeth Tamayo, Tamara Gómez-Gallego, Nuria Ferrol. Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast. Methods in molecular biology (Clifton, N.J.). 2020;2146:197-211

    Expand section icon Mesh Tags


    PMID: 32415605

    View Full Text
    • Copyright © 2021 Illumina Inc. All rights reserved.