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The obligate symbiotic nature of arbuscular mycorrhizal (AM) fungi makes extremely difficult their genetic manipulation or transformation. For this reason, a heterologous system has been traditionally used for functional analysis of AM fungal genes, being the budding yeast Saccharomyces cerevisiae an organism suitable for this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation, heterologous gene expression, functional complementation assays, preparation of yeast extracts, and subcellular localization of the encoded protein.

Citation

Elisabeth Tamayo, Tamara Gómez-Gallego, Nuria Ferrol. Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast. Methods in molecular biology (Clifton, N.J.). 2020;2146:197-211

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PMID: 32415605

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