Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens.

Nour Zahi Gammoh, Lothar Rink

Methods in molecular biology (Clifton, N.J.) 2020

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Conventional PCR techniques are laborious and usually not suited for fast screening of large sample numbers in a clinical or research setting. Using this closed-tube multiplex real-time PCR, the presence of all 11 Streptococcus pyogenes superantigen (SAg) genes can be rapidly and accurately characterized. Identifying whether a strain contains a SAg can be done within 4 h compared to conventional methods which would take 11 times as long. This method provides an excellent diagnostic tool as well as a screening tool to help researchers clarify the role of SAgs in S. pyogenes infections.

Citation

Nour Zahi Gammoh, Lothar Rink. Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens. Methods in molecular biology (Clifton, N.J.). 2020;2136:17-23