MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1.

Xinxin Li, Jiamin Qiu, Hehe Liu, Yan Deng, Shenqiang Hu, Jiwei Hu, Yushi Wang, Jiwen Wang

Bioscience reports 2020 Jun 26

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MiR-33a is found as a regulator of cell proliferation in many cancer cells. However, it remains unknown if and how miR-33a plays a role in myoblast proliferation. To investigate the effect of miR-33a on myoblast proliferation, miR-33a mimic or inhibitor was co-administered with or without insulin-like growth factor 1 (IGF1) to simulation myoblasts. Our study showed that up-regulation of miR-33a impaired myoblast proliferation, while down-regulation of miR-33a enhanced myoblast proliferation. Mechanistically, we examined that miR-33a can inhibit the transcription of IGF1, follistatin (FST) and cyclin D1 (CCND1) by targeting their 3'UTR region in both HEK293T cells and duck myoblasts. Moreover, up-regulation of miR-33a decreased and its down-regulation increased the mRNA expression of PI3K, Akt, mTOR and S6K. Importantly, the decreased PI3K, Akt, mTOR and S6K expression by miR-33a mimics was abrogated by co-administered with IGF1. Altogether, our results demonstrated that miR-33a may directly target IGF1, FST and CCND1 to inhibit myoblast proliferation via PI3K/Akt/mTOR signaling pathway. In conclusion, miR-33a is a potential negative regulator of myoblast proliferation and by modulating its expression could promote the early development of skeletal muscle. © 2020 The Author(s).

Citation

Xinxin Li, Jiamin Qiu, Hehe Liu, Yan Deng, Shenqiang Hu, Jiwei Hu, Yushi Wang, Jiwen Wang. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Bioscience reports. 2020 Jun 26;40(6)