Correlation Engine 2.0
Clear Search sequence regions

  • cell cycle (3)
  • dna neoplasm (2)
  • dye (3)
  • humans (1)
  • leukemia (1)
  • methyl green (6)
  • propidium (1)
  • stain (2)
  • Sizes of these terms reflect their relevance to your search.

    Methyl green (MG), a conventional, low-cost histological stain, was used to design a flow cytometric cell-cycle/DNA-ploidy assay. On fluorometry, MG absorbed maximally at 633-nm, showed negligible fluorescence in free-state, but emitted brightly when bound to DNA. Optimal dye and cell concentrations for staining and effects of time and photobleaching on stained cells were determined for a lyse-permeabilize-stain protocol. Linearity of DNA-binding, coefficients-of-variation of G0/G1-peaks and minimal carryover were confirmed. Assay results correlated highly with a propidium iodide-based kit in 29 acute lymphoblastic leukemia specimens. The MG-based DNA-ploidy assay represented an accurate and inexpensive alternative to conventional PI-based assays. Copyright © 2020 Elsevier Inc. All rights reserved.


    Pooja Murgai, Prashant Sharma, Man Updesh Singh Sachdeva, Parveen Lata Bose, Minakshi Gupta, Reena Das, Neelam Varma. A flow cytometric cell-cycle assay using methyl green. Analytical biochemistry. 2020 Jul 15;601:113782

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32450059

    View Full Text