New insights into ligand binding by plant lipid transfer proteins: A case study of the lentil Lc-LTP2.

D N Melnikova, I V Bogdanov, A A Ignatova, T V Ovchinnikova, E I Finkina

Biochemical and biophysical research communications 2020 Jul 12

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Lipid transfer proteins (LTPs) are an important class of plant proteins containing an internal cavity and binding hydrophobic ligands. Although LTP structures and functions are well studied, mechanisms of ligand binding remain unclear. Earlier, we discovered the lentil lipid transfer protein Lc-LTP2 capable of binding and transfer various ligands. We have shown that the "bottom" entrance of the Lc-LTP2 cavity takes part in attachment to the micelle surface and in lipids uptake. Here, we studied the role of Arg45 and Tyr80, located at the "bottom" entrance, in Lc-LTP2 ligand binding. We obtained recombinant mutant analogs of Lc-LTP2 (R45A, Y80A, R45A/Y80A), investigated their ability to bind fatty acids and lysolipids, as well as performed molecular modeling of the protein-ligand complexes. We showed that replacement of one or both residues led to a change of the internal hydrophobic cavity dimensions. As a result, lipids may change their orientation into the protein cavity, and thereby binding ability of mutant analogs may be affected as well. In the present work, we revealed an important role of Arg45 and Tyr80 in stabilization of the Lc-LTP2 complexes with both fatty acids and lysolipids with different ligand orientation. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

D N Melnikova, I V Bogdanov, A A Ignatova, T V Ovchinnikova, E I Finkina. New insights into ligand binding by plant lipid transfer proteins: A case study of the lentil Lc-LTP2. Biochemical and biophysical research communications. 2020 Jul 12;528(1):39-45