Dual-Mode Detection of Bacterial 16S Ribosomal RNA in Tissues.

The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, AI-Cz-Neo, is designed and synthesized by the conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that AI-Cz-Neo could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. These findings greatly expand the application of TADF fluorophores in time-resolved biological imaging and provide a promising strategy for the precise and reliable diagnosis of bacterial infections based on the dual-mode imaging of bacterial 16S rRNA by fluorescence intensity and fluorescence lifetime.

Citation

Shengnan Xu, Qingyang Zhang, Xiaowan Han, Yali Wang, Xiang Wang, Marc Nazare, Jian-Dong Jiang, Hai-Yu Hu. Dual-Mode Detection of Bacterial 16S Ribosomal RNA in Tissues. ACS sensors. 2020 Jun 26;5(6):1650-1656

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PMID: 32466642

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