Simultaneous determination of canrenone, digoxin and tolvaptan by UHPLC-MS/MS: application in heart failure patients.

Peng Guo, Qiucheng Liang, Juntao Zheng, Jinmin Chen, Yanping Guan, Liang Ding, Fulin Jiang, Xuewen Chen, Min Huang, Ailan Chen, Guoping Zhong

Bioanalysis 2020 May

filter terms:

Aim: Heart failure patients are frequently given comedication of digoxin and diuretics like spironolactone and tolvaptan. A UHPLC-MS/MS assay for determining canrenone (main active metabolite of spironolactone), digoxin and tolvaptan simultaneously should be developed so as to support related drug-drug interaction studies. Results: A UHPLC-MS/MS method for simultaneous determination of these three drugs in human plasma was established and fully verified as per CFDA guidelines. Chromatographic separation was achieved using a 4-min isocratic elution. Mass analyses were performed under positive electrospray ionization mode. The calibration curves were established over 1.0-400.0 ng/ml for canrenone and tolvaptan while over 0.1-40.0 ng/ml for digoxin. Conclusion: The developed method was feasible in detecting concentration and related drug-drug interaction studies.

Citation

Peng Guo, Qiucheng Liang, Juntao Zheng, Jinmin Chen, Yanping Guan, Liang Ding, Fulin Jiang, Xuewen Chen, Min Huang, Ailan Chen, Guoping Zhong. Simultaneous determination of canrenone, digoxin and tolvaptan by UHPLC-MS/MS: application in heart failure patients. Bioanalysis. 2020 May;12(9):569-582