Yuanyuan Guo, Jiao Zhang, Gaifang Pan, Chung Hang Jonathan Choi, Ping Wang, Yuehua Li, Xinyuan Zhu, Chuan Zhang
Chemical communications (Cambridge, England) 2020 Jul 11A multitude of maleimides are grafted onto the backbone of a phosphorothioate antisense oligonucleotide (ASO) to generate the construct of maleimide-grafted ASO (Mal-g-ASO). Through click conjugation with cell membrane thiols that triggers endocytosis-independent cellular internalization, Mal-g-ASO exhibited enhanced cellular uptake efficiency, resulting in a remarkable improvement of ASO-based gene silencing.
Yuanyuan Guo, Jiao Zhang, Gaifang Pan, Chung Hang Jonathan Choi, Ping Wang, Yuehua Li, Xinyuan Zhu, Chuan Zhang. Grafting multi-maleimides on antisense oligonucleotide to enhance its cellular uptake and gene silencing capability. Chemical communications (Cambridge, England). 2020 Jul 11;56(54):7439-7442
PMID: 32494799
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