Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes.

Shuang Yang, Kriti Bahl, Hui-Ting Chou, Jonathan Woodsmith, Ulrich Stelzl, Thomas Walz, Maxence V Nachury

eLife 2020 Jun 08

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Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base of cilia. Here, we present the near-atomic structures of the BBSome by itself and in complex with ARL6GTP, and we describe the changes in BBSome conformation induced by ARL6GTP binding. Modeling the interactions of the BBSome with membranes and the GPCR Smoothened (SMO) reveals that SMO, and likely also other GPCR cargoes, must release their amphipathic helix 8 from the membrane to be recognized by the BBSome. © 2020, Yang et al.

Citation

Shuang Yang, Kriti Bahl, Hui-Ting Chou, Jonathan Woodsmith, Ulrich Stelzl, Thomas Walz, Maxence V Nachury. Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes. eLife. 2020 Jun 08;9