NAP1-RELATED PROTEIN1 and 2 negatively regulate H2A.Z abundance in chromatin in Arabidopsis.

Yafei Wang, Zhenhui Zhong, Yaxin Zhang, Linhao Xu, Suhua Feng, Shima Rayatpisheh, James A Wohlschlegel, Zonghua Wang, Steven E Jacobsen, Israel Ausin

Nature communications 2020 Jun 08

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In eukaryotes, DNA wraps around histones to form nucleosomes, which are compacted into chromatin. DNA-templated processes, including transcription, require chromatin disassembly and reassembly mediated by histone chaperones. Additionally, distinct histone variants can replace core histones to regulate chromatin structure and function. Although replacement of H2A with the evolutionarily conserved H2A.Z via the SWR1 histone chaperone complex has been extensively studied, in plants little is known about how a reduction of H2A.Z levels can be achieved. Here, we show that NRP proteins cause a decrease of H2A.Z-containing nucleosomes in Arabidopsis under standard growing conditions. nrp1-1 nrp2-2 double mutants show an over-accumulation of H2A.Z genome-wide, especially at heterochromatic regions normally H2A.Z-depleted in wild-type plants. Our work suggests that NRP proteins regulate gene expression by counteracting SWR1, thereby preventing excessive accumulation of H2A.Z.

Citation

Yafei Wang, Zhenhui Zhong, Yaxin Zhang, Linhao Xu, Suhua Feng, Shima Rayatpisheh, James A Wohlschlegel, Zonghua Wang, Steven E Jacobsen, Israel Ausin. NAP1-RELATED PROTEIN1 and 2 negatively regulate H2A.Z abundance in chromatin in Arabidopsis. Nature communications. 2020 Jun 08;11(1):2887