Snake venom characteristic peptides: novel fingerprints for species identification by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry.

Ying Liu, Xiao-Hui Zhang, Yue Yu, Hong-Xu Chen, Ying-Lin Zhou, Xin-Xiang Zhang

The Analyst 2020 Jul 21

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Snake venom is a complex mixture mainly consisting of proteins and peptides which varies with different species. These variations lead to different toxic mechanisms and different anti-venom serums for treatment and the determination of their use as drugs. Hence, it is important to develop a sensitive and reliable method to identify the species of snakes from venoms. Herein, we present a novel strategy based on the sheathless capillary electrophoresis-electrospray ionization-mass spectrometry (CESI-MS) system to characterize snake venom proteins. Through the determination of peptides, we found the characteristic peptides of 8 different snakes with high sensitivity (1 μg mL-1) and high selectivity, which provided a reliable method for the species identification and purity detection of snake venom samples.

Citation

Ying Liu, Xiao-Hui Zhang, Yue Yu, Hong-Xu Chen, Ying-Lin Zhou, Xin-Xiang Zhang. Snake venom characteristic peptides: novel fingerprints for species identification by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry. The Analyst. 2020 Jul 21;145(14):5027-5031