Blood circulation of soft nanomaterials is governed by dynamic remodeling of protein opsonins at nano-biointerface.

Srinivas Abbina, Lily E Takeuchi, Parambath Anilkumar, Kai Yu, Jason C Rogalski, Rajesh A Shenoi, Iren Constantinescu, Jayachandran N Kizhakkedathu

Nature communications 2020 Jun 16

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Nanomaterials in the blood must mitigate the immune response to have a prolonged vascular residency in vivo. The composition of the protein corona that forms at the nano-biointerface may be directing this, however, the possible correlation of corona composition with blood residency is currently unknown. Here' we report a panel of new soft single molecule polymer nanomaterials (SMPNs) with varying circulation times in mice (t1/2β ~ 22 to 65 h) and use proteomics to probe protein corona at the nano-biointerface to elucidate the mechanism of blood residency of nanomaterials. The composition of the protein opsonins on SMPNs is qualitatively and quantitatively dynamic with time in circulation. SMPNs that circulate longer are able to clear some of the initial surface-bound common opsonins, including immunoglobulins, complement, and coagulation proteins. This continuous remodelling of protein opsonins may be an important decisive step in directing elimination or residence of soft nanomaterials in vivo.

Citation

Srinivas Abbina, Lily E Takeuchi, Parambath Anilkumar, Kai Yu, Jason C Rogalski, Rajesh A Shenoi, Iren Constantinescu, Jayachandran N Kizhakkedathu. Blood circulation of soft nanomaterials is governed by dynamic remodeling of protein opsonins at nano-biointerface. Nature communications. 2020 Jun 16;11(1):3048