Cis-2 and trans-2-eisocenoic fatty acids are novel inhibitors for Mycobacterium tuberculosis Protein tyrosine phosphatase A.

Lalu Rudyat Telly Savalas, Baiq Repika Nurul Furqon, Dina Asnawati, Jannatin 'Ardhuha, Prapti Sedijani, Saprizal Hadisaputra, Baiq Nila Sari Ningsih, Jufrizal Syahri

Acta biochimica Polonica 2020 Jun 18

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Small protein tyrosine phosphatase (PtpA) of Mycobacterium tuberculosis is attributed to the development of latent tuberculosis infection, and hence bocomes an interesting target for drug development. In this communication, inhibition of PtpA by naturally occurring fatty acids cis-2 and trans-2-eicosenoic acid is investigated. Mtb PtpA was heterologously expressed in Escherichia coli, and the activity of PtpA was inhibited by cis-2 and trans-2 eicosenoic fatty acids. Both compunds showed strong inhibition of PtpA activity with IC50 at low micromolar concentration. As comparison, trans-11-eicosenoic acid only slightly inhibit PtpA. In silico analysis confirmed the inhibition of PtpB by cis-2-eicosenoic acid by formation of several hydrogen bonds. These findings show that cis-2 and trans-2 eicosenoic fatty acids are potential candidates for latent tuberculosis inhibitors.

Citation

Lalu Rudyat Telly Savalas, Baiq Repika Nurul Furqon, Dina Asnawati, Jannatin 'Ardhuha, Prapti Sedijani, Saprizal Hadisaputra, Baiq Nila Sari Ningsih, Jufrizal Syahri. Cis-2 and trans-2-eisocenoic fatty acids are novel inhibitors for Mycobacterium tuberculosis Protein tyrosine phosphatase A. Acta biochimica Polonica. 2020 Jun 18;67(2):219-223