Structural analyses of the HG-type pectin from notopterygium incisum and its effects on galectins.

Mengshan Zhang, Heyang Zu, Xumin Zhuang, Yang Yu, Yuan Wang, Zihan Zhao, Yifa Zhou

International journal of biological macromolecules 2020 Nov 01

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In the manuscript, water-soluble polysaccharides WPNI was extracted from notopterygium incisum roots and separated into two homogeneous fractions WPNI-A-a and WPNI-A-b. WPNI-A-a was an arabinogalactan (AG). WPNI-A-b belonged to HG type pectin. The structure of WPNI-A-b was analyzed by FT-IR, NMR, enzymatic hydrolysis (Endo-PG) and UPLC-FLD-MSn. WPNI-A-b was dominated by HG domain, covalently linked with AG and RG-II domains. Oligogalacturonides produced by Endo-PG from HG domain were non-, mono-, di- or tri-methyl esterified with degree of polymerization (DP) from 1 to 6. The distribution of methyl-ester groups was in a block-wise manner. The interaction of WPNI-A-b and its enzymatic hydrolysis products with galectin-1, galectin-3, galectin-7 and galectin-8 showed that AG domain exhibited stronger binding avidity to galectins than RG-II and HG domain, while oligogalacturonides showed no binding activities to galectins. The results would be useful for the application of the pectin from notopterygium incisum. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Mengshan Zhang, Heyang Zu, Xumin Zhuang, Yang Yu, Yuan Wang, Zihan Zhao, Yifa Zhou. Structural analyses of the HG-type pectin from notopterygium incisum and its effects on galectins. International journal of biological macromolecules. 2020 Nov 01;162:1035-1043