Kupffer Cell Protein Isolation and Detection by Western Blot.

Valerio Azzimato

Methods in molecular biology (Clifton, N.J.) 2020

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Kupffer cells are the majority of tissue macrophages present in the body and are actively involved in the regulation of liver homeostasis as well as in the progression of liver diseases. Here we describe a methodology to successfully isolate and detect proteins from Kupffer cells and avoid the contamination of other liver cell populations.