Papain immobilized on alginate membrane for wound dressing application.

Raimundo Nonato Fernandes Moreira Filho, Niédja Fittipaldi Vasconcelos, Fabia Karine Andrade, Morsyleide de Freitas Rosa, Rodrigo Silveira Vieira

Colloids and surfaces. B, Biointerfaces 2020 Oct

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Wound dressings based on natural polymers are of considerable interest in the pharmaceutical industry owing to their improved performance in the human body when compared to synthetic polymers. Alginate, a polysaccharide from brown algae, is commonly studied as a wound dressing owing to its biocompatibility and biodegradability. To improve its therapeutic features and thereby increase wound healing, papain (a proteolytic enzyme from Carica papaya latex) was proposed to be incorporated. Papain is capable of promoting the debridement of devitalized or necrotic tissues. The development of dressing based on alginate and papain aggregates the healing properties of both materials. In addition, the adsorption on a support can stabilize the enzyme structure and permits its release in a controlled manner. The optimal conditions for immobilization were evaluated (initial concentration, temperature, and pH), and the amount immobilized was measured by Bradford assay. The enzyme activity stability over 28 days was measured. The release profile was determined using Franz cell. In vitro cytotoxicity assays were performed using fibroblasts and keratinocytes. Optimal immobilization conditions were identified in a neutral medium at a papain concentration of 20 mg/mL and temperature of 25 °C. The enzyme remained active after immobilization (80 % of its initial activity), and the matrix protected the enzyme from deactivation (70 % reduction on the matrix compared to 94 % in a buffer solution). Franz cell displayed a release profile of 64.1 % of the enzyme after 24 h. The biological assays indicated a bioactive material with proteolytic properties. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Raimundo Nonato Fernandes Moreira Filho, Niédja Fittipaldi Vasconcelos, Fabia Karine Andrade, Morsyleide de Freitas Rosa, Rodrigo Silveira Vieira. Papain immobilized on alginate membrane for wound dressing application. Colloids and surfaces. B, Biointerfaces. 2020 Oct;194:111222