Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform.

Kathleen Van Manen-Brush, Jacob Zeitler, John R White, Paul Younge, Samantha Willis, Marisa Jones

BioTechniques 2020 Sep

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Chinese hamster ovary (CHO) cells are a mammalian cell line used in the production of therapeutic proteins. Host cell proteins (HCPs) are process-related impurities that are derived from the host cell expression system. During biopharmaceutical drug development, removal of HCPs is required. Enzyme-linked immunosorbent assay (ELISA) is a common technique to quantitate HCPs, but is a labor-intensive process that takes up to 7 h. Ella® is an automated instrument that utilizes microfluidics and glass nanoreactors to quantitate HCPs in 75 min using similar ELISA reagents. The antibodies and antigens are captured on three distinct glass nanoreactors, resulting in sensitive reproducible data. Our results indicate that Ella quantitates CHO HCPs with precision, accuracy, sensitivity and trends comparable with our traditional CHO HCP ELISA.

Citation

Kathleen Van Manen-Brush, Jacob Zeitler, John R White, Paul Younge, Samantha Willis, Marisa Jones. Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform. BioTechniques. 2020 Sep;69(3):186-192