In vivo mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly.

Herein, we develop a novel tripartite DNA probe to transport phosphorothioated substrate hairpins and an aptamer for the intramolecular CHA circuit, which achieves detection of a low amount of specific mRNA in living cells and mice. Our study provides an improved strategy to promote in vivo fluorescence imaging applications in early-stage clinical diagnosis.

Citation

Han Wu, Wen-Jing Zhou, Lan Liu, Ze Fan, Hao Tang, Ru-Qin Yu, Jian-Hui Jiang. In vivo mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly. Chemical communications (Cambridge, England). 2020 Aug 04;56(62):8782-8785

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PMID: 32618290

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