Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries.

Xueting Fu, Haihong Yang, Conglei Ma, Xiaoqing Li, Dalong Li, Yu Yang, Yaqin Xu, Libo Wang

International journal of biological macromolecules 2020 Nov 15

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An acidic heteropolysaccharide HEP-2 was obtained from blue honeysuckle berry extracts after purification using Sepharose 6B and Sephadex G-200 column chromatography. The molecular weight of HEP-2, determined with high-performance gel permeation chromatography, was 3.01 × 106 Da. Fourier transform infrared spectroscopy, gas chromatography and nuclear magnetic resonance analyses revealed the presence of α-, β-pyranose ring and six sugar residues in HEP-2. Amorphous aggregates of HEP-2 with irregular shape and lacking the triple helical structure were detected using the Congo red test, scanning electron and atomic force microscopy, and X-ray diffraction studies. Rheological characterization showed typical shear-thinning behavior and viscoelastic property of the polysaccharide. HEP-2 exerted significant inhibitory effects on α-amylase and α-glucosidase and displayed competitive inhibition kinetics. The collective results support the potential utility of HEP-2 as a hypoglycemic agent for enzyme-targeted treatment of diabetes or functional food. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Xueting Fu, Haihong Yang, Conglei Ma, Xiaoqing Li, Dalong Li, Yu Yang, Yaqin Xu, Libo Wang. Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries. International journal of biological macromolecules. 2020 Nov 15;163:414-422