Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography.

Ye Yuan, Xiaoping He, Tingting Wang, Xingwang Zhang, Zhong Li, Xiaoqing Xu, Weiyan Zhang, Xiaojun Yan, Shengying Li, Shan He

Marine drugs 2020 Jun 25

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An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid-liquid extraction (LLE) with a three-phase solvent system (n-hexane-ethyl acetate-acetonitrile-water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane-acetonitrile-water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract.

Citation

Ye Yuan, Xiaoping He, Tingting Wang, Xingwang Zhang, Zhong Li, Xiaoqing Xu, Weiyan Zhang, Xiaojun Yan, Shengying Li, Shan He. Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography. Marine drugs. 2020 Jun 25;18(6)