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A toolbox for chemotaxis assay adapted to Leptospira spp. has emerged in the recent years: soft agar assay, capillary assay, and videomicroscopy tracking. Those methods allow to demonstrate chemotaxis defect, identify diverse chemoattractants, or decipher motile behavior quantitatively. These experiments have demonstrated a role of motility and potentially chemotaxis in leptospirosis pathogenesis. We describe extensively the methods and provide the key steps to use this toolbox.


Ambroise Lambert. Leptospira spp. Toolbox for Chemotaxis Assay. Methods in molecular biology (Clifton, N.J.). 2020;2134:123-130

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PMID: 32632864

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