Neto-α Controls Synapse Organization and Homeostasis at the Drosophila Neuromuscular Junction.

Tae Hee Han, Rosario Vicidomini, Cathy Isaura Ramos, Qi Wang, Peter Nguyen, Michal Jarnik, Chi-Hon Lee, Michal Stawarski, Roberto X Hernandez, Gregory T Macleod, Mihaela Serpe

Cell reports 2020 Jul 07

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Glutamate receptor auxiliary proteins control receptor distribution and function, ultimately controlling synapse assembly, maturation, and plasticity. At the Drosophila neuromuscular junction (NMJ), a synapse with both pre- and postsynaptic kainate-type glutamate receptors (KARs), we show that the auxiliary protein Neto evolved functionally distinct isoforms to modulate synapse development and homeostasis. Using genetics, cell biology, and electrophysiology, we demonstrate that Neto-α functions on both sides of the NMJ. In muscle, Neto-α limits the size of the postsynaptic receptor field. In motor neurons (MNs), Neto-α controls neurotransmitter release in a KAR-dependent manner. In addition, Neto-α is both required and sufficient for the presynaptic increase in neurotransmitter release in response to reduced postsynaptic sensitivity. This KAR-independent function of Neto-α is involved in activity-induced cytomatrix remodeling. We propose that Drosophila ensures NMJ functionality by acquiring two Neto isoforms with differential expression patterns and activities. Published by Elsevier Inc.

Citation

Tae Hee Han, Rosario Vicidomini, Cathy Isaura Ramos, Qi Wang, Peter Nguyen, Michal Jarnik, Chi-Hon Lee, Michal Stawarski, Roberto X Hernandez, Gregory T Macleod, Mihaela Serpe. Neto-α Controls Synapse Organization and Homeostasis at the Drosophila Neuromuscular Junction. Cell reports. 2020 Jul 07;32(1):107866