Perspectives for the microbial production of ethyl acetate.

Shangjie Zhang, Feng Guo, Wei Yan, Weiliang Dong, Jie Zhou, Wenming Zhang, Fengxue Xin, Min Jiang

Applied microbiology and biotechnology 2020 Sep

filter terms:

Ethyl acetate is one of the short-chain esters and widely used in the food, beverage, and solvent areas. The ethyl acetate production currently proceeds through unsustainable and energy intensive processes, which are based on natural gas and crude oil. Microbial conversion of biomass-derived sugars into ethyl acetate may provide a sustainable alternative. In this review, the perspectives of bio-catalyzing ethanol and acetic acid to ethyl acetate using lipases in vitro was introduced. Besides, the crucial elements for high yield of ethyl acetate in fermentation was expounded. Also, metabolic engineering in yeasts to product ethyl acetate in vivo using alcohol acyl transferases (AAT) was discussed. KEY POINTS: •The accumulation of acetyl-CoA is crucial for synthesizing ethyl acetate in vivo; AAT-mediated metabolic engineering could efficiently improve ethyl acetate production.

Citation

Shangjie Zhang, Feng Guo, Wei Yan, Weiliang Dong, Jie Zhou, Wenming Zhang, Fengxue Xin, Min Jiang. Perspectives for the microbial production of ethyl acetate. Applied microbiology and biotechnology. 2020 Sep;104(17):7239-7245