Recent Development in Ligation Methods for Glycopeptide and Glycoprotein Synthesis.

Glycoproteins are produced by the post-translational modification process of proteins and they play an important role in mediating various biological processes. Our understanding towards biochemical functions of individual glycoproteins has been seriously hampered due to the heterogeneous expression of carbohydrate parts in glycoproteins. Despite the advancement in recombinant expression and chromatographic techniques, the isolation of pure glycoforms remains nearly impossible. To obtain homogenous glycoproteins, tremendous efforts hves been spent in developing various ligation and glycosylation techniques. This minireview discusses selected methods for the preparation and ligation of glycopeptides. The importance of the development of new chemical synthesis method for glycoproteins has also been discussed, which would be one of the next directions in this field. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Junjie Desmond Lin, Xue-Wei Liu. Recent Development in Ligation Methods for Glycopeptide and Glycoprotein Synthesis. Chemistry, an Asian journal. 2020 Sep 01;15(17):2548-2557

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PMID: 32657034

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