A two-lane mechanism for selective biological ammonium transport.

The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH4+ transport in two archetypal members of the family, the transporters AmtB from Escherichia coli and Rh50 from Nitrosomonas europaea. The pathway underpins a mechanism by which charged H+ and neutral NH3 are carried separately across the membrane after NH4+ deprotonation. This mechanism defines a new principle of achieving transport selectivity against competing ions in a biological transport process. © 2020, Williamson et al.

Citation

Gordon Williamson, Giulia Tamburrino, Adriana Bizior, Mélanie Boeckstaens, Gaëtan Dias Mirandela, Marcus G Bage, Andrei Pisliakov, Callum M Ives, Eilidh Terras, Paul A Hoskisson, Anna Maria Marini, Ulrich Zachariae, Arnaud Javelle. A two-lane mechanism for selective biological ammonium transport. eLife. 2020 Jul 14;9

Mesh Tags

Substances

PMID: 32662768

search → result