Variability and additivity of read counts for aSTRs in NGS DNA profiles.

There has been an increase in the number of laboratories and researchers adopting new sequencing technologies, known as next-generation sequencing (NGS). An understanding of the behaviour of NGS DNA profiles is needed to enable for the development of probabilistic genotyping methods for the interpretation of such profiles. In this work, we investigate NGS analyte signal variation, specifically heterozygous balance and stutter variability from profiles generated using the ForenSeq™ DNA Signature Prep Kit, DNA Primer Mix B. We also investigate additivity of analyte signals in NGS profiles for overlapping allelic and stutter signals originating from the same or different contributors. We describe models that can be used to inform a continuous method for the interpretation of DNA profiling data. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Kevin Cheng, Jessica Skillman, Stephanie Hickey, Rebecca Just, Lilliana Moreno, Jo-Anne Bright, Hannah Kelly, Meng-Han Lin, James M Curran, John Buckleton. Variability and additivity of read counts for aSTRs in NGS DNA profiles. Forensic science international. Genetics. 2020 Sep;48:102351

Mesh Tags

PMID: 32682320

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