Ethylene glycol and glycolic acid production by wild-type Escherichia coli.

Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-type Escherichia coli W3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore, E. coli strains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced by E. coli ΔyqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, the E. coli ΔaldA+yqhD produced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production by E. coli on xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization. © 2020 International Union of Biochemistry and Molecular Biology, Inc.

Citation

Xiyang Lu, Yao Yao, Yang Yang, Zhongxi Zhang, Jinjie Gu, Ljiljana Mojovic, Zorica Knezevic-Jugovic, Frank Baganz, Gary Lye, Jiping Shi, Jian Hao. Ethylene glycol and glycolic acid production by wild-type Escherichia coli. Biotechnology and applied biochemistry. 2021 Aug;68(4):744-755

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PMID: 32683722

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